The Glow Worms are located in an old rail tunnel 400 metres in length. It was originally constructed as part of the railway line to Newnes Kerosene Shale Works. The line was dismantled around the 1940s.The tunnel is located 40 kilometres from Lithgow on the Newnes Plateau. Good walking shoes and a torch are required for the walk through the tunnel.
The glow worms found in the tunnel are the larvae of the fungus gnat. The blue glow of the larvae is the result of a chemical reaction in the body of the glow worm. The larvaes' glow lures their prey, such as mosquito's, closer to them.